Rapid Genetic Assessment of Carrot Varieties Based on AFLP Analysis

It is necessary to regard the biodiversity of carrot as a genetic source useful and indispensable components for human diet. Amplified fragment length polymorphism (AFLP) markers were used discriminate eight genotypes with different root colors. With combination enzymes Tru9I, PstI, three sets primer pairs corresponding adapters joint restricted sites, 92 loci produced, including 60 polymorphic ones. Each showed high efficiency, according estimations PIC (0.34, 0.34, 0.41), D (0.36, 0.67, 0.67), Rp (5.5, 11.3, 15), HE (0.32, 0.49, 0.49). The distances calculated using values Nei’s coefficient. most genetically similar Chantenay Coeur Rouge Colmar at distance 0.12, whereas distant Saint Valery Purple Dragon highest 0.34. Confirming its identity, Gelber Goliath purple yellow roots detached varieties from others 0.23–0.34 0.23–0.28, respectively. Male sterile Berlicum breeding accessions well distinct other orange-colored 0.30 Deep F1. Slight modifications facilitation gel staining enables wide use AFLP method diversity assessment in accessions.